ABSTRACT
Simple and effective high performance liquid chromatographic [HPLC] method was developed for estimation of Clindipine in drug free human drug free blank plasma. The internal standard used as Nifidipine [IS]. The current method was used protein precipitating extraction of Clindipine from blank plasma. Separation was achieved on reversedphase c[18] column [25cm × 4.6mm, 5 micro] and the detection was monitored by UV detector at 260 nm. The optimized mobile phase was used acetonitrile: 5mM potassium dihydrogen orthophosphate [pH 4.5], in the ratio of 60:40% v/v at a flow rate of 1.0 ml/min. This linearity was achieved in this method range of 10.0-125.0 ng/ml with regression coefficient range is 0.99. The present method is suitable in terms of precise, accurate and specific during the study. The simplicity of the method allows for application in laboratories that lack sophisticated analytical instruments such as LC-MS/MS or GC-MS/MS that are complicated, costly and time consuming rather than a simple HPLC-UV method. The present method was successfully applied for pharmacokinetic studies